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  2. Genetics 101
  3. Week 7-9
  4. Molecular Genetics Continuation & Bioinformatics Tools
  5. Lab Simulation – Gene Sequence Comparison using BLAST

Lab Simulation – Gene Sequence Comparison using BLAST

Completion requirements
Opened: Tuesday, 20 May 2025, 12:00 AM
Due: Tuesday, 27 May 2025, 12:00 AM

Simulation Tool:

NCBI BLAST (Basic Local Alignment Search Tool)
🔗 https://blast.ncbi.nlm.nih.gov/Blast.cgi


Instructions:

  1. Go to the BLAST homepage: https://blast.ncbi.nlm.nih.gov/Blast.cgi

  2. Click on “nucleotide BLAST” under the “Basic BLAST” section.

  3. In the “Enter Query Sequence” box, paste the following sample DNA sequence:

     
    ATGGAATTCAAGGAGATATAACAATGGCAGGAAGATGAGGAGGACGAAGAAGCAGGAGTGGAGGAAGAGG
  4. Under “Database”, select:

    • “Nucleotide collection (nr/nt)”

  5. Leave all other settings as default, and click “BLAST” at the bottom.

  6. Once the results are generated:

    • Scroll to the Descriptions and Alignments sections.

    • Identify the organism from which the top hit is derived.

    • Record the % identity, E-value, and alignment length.


Student Task:

Submit a short lab report that includes:

  • A screenshot of your BLAST results.

  • A summary of the organism matched, with:

    • Scientific name

    • % identity

    • E-value

    • Function of the matched gene (if available)

  • A short reflection (100–150 words) on how BLAST can be useful in genetics and medicine.


Submission Instructions:

  • Upload as: PDF or Word document

  • File Name Format: YourName_BLAST_Report

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